Tzeni Seltsa graduated from the School of Veterinary Medicine of Aristotle University of Thessaloniki in 2014. From 2014 to 2016, she worked as a postgraduate trainee veterinarian at the Companion Animal Surgery, Anesthesiology and Intensive Care Unit of the School of Veterinary Medicine of Aristotle University of Thessaloniki, where she also completed her postgraduate studies and obtained an MSc in Companion Animal Surgery. She has also attended the ESAVS (European School for Advanced Veterinary Studies) program in Companion Animal Orthopedics.

Since 2016, she has been working as a surgeon at a veterinary clinic in Thessaloniki, while also working as an external collaborating surgeon at veterinary clinics and practices, performing surgical procedures.

She has attended educational seminars in Greece and abroad focusing on Companion Animal Surgery and Orthopedics, as well as diagnostic imaging and evaluation of hip dysplasia. She has participated in nationwide scientific conferences, both as an attendee and as a speaker, and has published scientific papers in international scientific journals.